| F1+2

Prothrombin fragment 1+2 (F 1+2) is the amino terminal fragment of the prothrombin molecule. When the thrombin is converted to thrombin by the thrombin complex, F 1+2 is released from the thrombin, which is a polypeptide with a half-life of about 9 minutes. Thrombin activation is carried out in the presence of factor Xa, factor Va, calcium ions and phospholipid surfaces (platelets). During thrombin formation, the prothrombin fragment F1+2 can be produced in two ways.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	200	100	50	25	12.5	6.25	3.12	0
OD	2.61	2.12	1.36	0.79	0.44	0.27	0.21	0.09
Corrected OD	2.52	2.03	1.27	0.7	0.35	0.18	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat F1+2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Plasma(n=8)	93-106	102

 

| Linearity

Samples were spiked with high concentrations of Rat F1+2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Plasma(n=4)
1 : 2	Range (%)	88-96
	Average (%)	93
1 : 4	Range (%)	87-108
	Average (%)	97